Regulatory
p2001

Part:BBa_K1820002

Designed by: Thomas Overbeck   Group: iGEM15_Utah_State   (2015-09-16)

P335-Φ31phage genetic switch-divergent promoter

A divergent phage-inducible promoter from the genetic switch region of the P335 species lactococcal phage Φ31.

Usage and Biology

This promoter region contains a leftward and rightward promoter. Expression is induced from the rightward promoter upon initial phage infection, resulting in transcription of the downstream genes.

This construct has yet to be cloned and expressed in L. lactis, and validated for functionality.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Durmaz E, Madsen SM, Israelsen H, Klaenhammer TR (2002) Lactococcus lactis lytic bacteriophages of the P335 group are inhibited by overexpression of a truncated CI repressor. J Bacteriol 184:6532–6544.

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